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Assay & Elisa Kits
ELISA Kit DIY Materials for Alpha-2-Macroglobulin (a2M)
tcue60069
ELISA Kit DIY Materials for Alpha-2-Macroglobulin (a2M)
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AVAILABLE SIZES
5X96 Tests
10X96 Tests
20x96 Tests
$
0.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
Data sheet
Download PDF Datasheet
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
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Test Principle
Double-antibody Sandwich ELISA for Antigen Detection
Information
Application
Main materials for "Do It (ELISA Kit) Yourself"
Species Reactivity
Homo sapiens (Human)
Sample Type
Serum, plasma, urine and other biological fluids.
Specifications
Sensitivity
0.35ng/mL
Detection Range
0.78-50ng/mL
Component
Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Misc Information
Storage Instruction
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
Notes
The Abs in the kit have high sensitivity and excellent specificity for detection of Alpha-2-Macroglobulin (a2M).No significant cross-reactivity or interference between Alpha-2-Macroglobulin (a2M) and analogues was observed.
Protocol
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
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