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Assay & Elisa Kits
ELISA Kit DIY Materials for Apolipoprotein L (APOL1)
tcue60026
ELISA Kit DIY Materials for Apolipoprotein L (APOL1)
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AVAILABLE SIZES
5X96 Tests
10X96 Tests
20x96 Tests
$
0.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
Data sheet
Download PDF Datasheet
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
Treatment
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Acetone
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Heat mediated
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Test Principle
Double-antibody Sandwich ELISA for Antigen Detection
Information
Application
Main materials for "Do It (ELISA Kit) Yourself"
Species Reactivity
Human
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Specifications
Sensitivity
0.51ng/mL
Detection Range
1.25-80ng/mL
Misc Information
Storage Instruction
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C.
TMB should be stored at 4°C.
96-well Plate could be stored at room temperature.
The contents are valid for twelve months.
They are stable for one month after opening when stored at 4°C.
Protocol
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
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