tcrk9339 Clonbit- EDTA-free Protease Inhibitor Cocktail

Product Description

EDTA-free Protease Inhibitor Tablets, inhibit a broad spectrum of serine and cysteine proteases.It has been used as a component of radioimmunoprecipitation assay (RIPA) lysis buffer for resuspending Chinese hamster ovary (CHO) cells.EDTA-free Protease Inhibitor Cocktail also has been used as as a component of:

• Immunoprecipitation lysis buffer (IPLS) for lysis of human embryonic kidney cells 293 (HEK293T cells) for glutathione co-precipitation.

• Adioimmunoprecipitation assay (RIPA) buffer for lysis of cells for western blotting

• Buffer A for the lysis of bacterial cells.

Information

ApplicationProtease Inhibitor Cocktails.

Research AreaEnzyme inhibitor.

Specifications

FormTablet

Recommended DilutionSoluble in aqueous buffers
or add directly to extraction media. Alternatively
prepare 25x stock solutions in 2 ml water or 100 mM phosphate buffer pH 7.0.
Stock solution is stable for 1-2 weeks at 2 to 8 °C or at least 12 weeks at -15 to -25 °C
Can be used in thiol-containing solutions at 15 to 25 °C.

SolubilityAqueous buffer: soluble

Misc Information

Storage InstructionThe stock solution can be stored at 2 to 8 °C for 1 to 2 weeks, or at least 12 weeks at -15 to -25 °C.

NotesAbsorption: 0.08 at 280 nm

tcrk9339 Clonbit- EDTA-free Protease Inhibitor Cocktail

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Product Description

Information

Specifications

Misc Information