tcrk9339 Clonbit- EDTA-free Protease Inhibitor Cocktail

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Product Description

EDTA-free Protease Inhibitor Tablets, inhibit a broad spectrum of serine and cysteine proteases.It has been used as a component of radioimmunoprecipitation assay (RIPA) lysis buffer for resuspending Chinese hamster ovary (CHO) cells.EDTA-free Protease Inhibitor Cocktail also has been used as as a component of:

• Immunoprecipitation lysis buffer (IPLS) for lysis of human embryonic kidney cells 293 (HEK293T cells) for glutathione co-precipitation.

• Adioimmunoprecipitation assay (RIPA) buffer for lysis of cells for western blotting

• Buffer A for the lysis of bacterial cells.


ApplicationProtease Inhibitor Cocktails.
Research AreaEnzyme inhibitor.


Recommended DilutionSoluble in aqueous buffers
or add directly to extraction media. Alternatively
prepare 25x stock solutions in 2 ml water or 100 mM phosphate buffer pH 7.0.
Stock solution is stable for 1-2 weeks at 2 to 8 °C or at least 12 weeks at -15 to -25 °C
Can be used in thiol-containing solutions at 15 to 25 °C.
SolubilityAqueous buffer: soluble

Misc Information

Storage InstructionThe stock solution can be stored at 2 to 8 °C for 1 to 2 weeks, or at least 12 weeks at -15 to -25 °C.
NotesAbsorption: 0.08 at 280 nm
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