tcel564 RPMI 8226

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Product Description
There is no evidence of heavy chain production(cytoplasmic or secreted).

Information
TissueHuman peripheral blood
Morphologylymphoblast
Growth Propertiessuspension

Specifications
Complete Growth MediumRPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio1:2-1:4
Medium Renewalevery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium＋30% FBS＋10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Antigen ExpressionHLA Aw19, B15, B37, Cw2; CD19 -; CD20 -; CD28＋; CD38＋; CD49e＋
Gene Expressionimmunoglobulin light chain

Misc Information
SubculturingCultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:3-1:5.
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