Product Description
The cell line is dependent on the presence of recombinant IL-2 and a dose as low as 10 U/mL is sufficient to maintain proliferation; cells will die within 72 hours in the absence of IL-2. NK-92 cells have the following characteristics: surface marker positive for CD2, CD7, CD11a, CD28, CD45, CD54 and CD56 bright; surface marker negative for CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19, CD20, CD23, CD34 and HLA-DR. ATCC confirmed this cell line is positive for the presence of Epstein-Barr viral DNA sequences via PCR.
Information
Applicationtransfection and can be used effectively for immunological ex vivo purging of leukemia from blood without compromising hematopoietic cell function.
TissueHuman peripheral blood
Morphologylymphoblast
Growth Propertiessuspension, multicell aggregates
Specifications
Complete Growth MediumMEMα (PM150421)+0.2mM Inositol+0.1mM β-mercaptoethanol (PB180633)+0.02mM Folic Acid+100-200U/mL recombinant IL-2+12.5% HS (164215-100)+12.5% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio1:2-1:4
Medium Renewalevery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium＋30% FBS＋10% DMSO
Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5%
Temperature: 37℃
Antigen ExpressionCD2＋, CD7＋, CD11a＋, CD28＋, CD45＋, CD54＋, CD56＋, CD1 -, CD3 -, CD4 -, CD5 -, CD8 -, CD10 -, CD14 -, CD16 -, CD19 -, CD20 -, CD23 -, CD34 -, HLA- DR -
Durationtransfection and can be used effectively for immunological ex vivo purging of leukemia from blood without compromising hematopoietic cell function.
Misc Information
SubculturingCultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.