Product Description
The depositor reports that the RWPE-1 cell line, derived from the same prostate, was screened for Hepatitis B and C, and human immunodeficiency viruses, and was found to be negative.
Information
ApplicationBecause of their derivation from the same peripheral zone of the prostate, the WPMY-1 cell line is especially useful for studies on paracrine and stromal(epithelial interactions).
TissueHuman prostate/stroma
Morphologymyofibroblast
Growth Propertiesadherent
EffectsNo, in nude mice(at Passage 22 cells did not form tumors when injected subcutaneously)
Yes, the cells form colonies in soft agar(colony forming efficiency of 0.7%).
Specifications
Complete Growth MediumDMEM (PM150210)+5% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio1:2-1:4
Medium Renewalevery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium＋30% FBS＋10% DMSO
Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5%
Temperature: 37℃
TumorigenicNo
Receptor Expressionandrogen receptor, expressed
Antigen Expressionkallikrein 3, KLK3(prostate specific antigen, PSA); Homo sapiens
Gene Expressionfibronectin; smooth muscle alpha-actin; vimentin
DurationBecause of their derivation from the same peripheral zone of the prostate, the WPMY-1 cell line is especially useful for studies on paracrine and stromal(epithelial interactions).
Misc Information
SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor.
Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.