tcel301 M-NFS-60 [NFS-60]

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Product Description
The cells are responsive to both interleukin 3(interleukin-3, IL-3) and macrophage colony stimulating factor(M-CSF).The cells contain a truncated c-myb proto-oncogene caused by integration of a retrovirus.

Information
TissueMouse bone marrow
Morphologylymphoblast
Growth Propertiessuspension

Specifications
Complete Growth MediumRPMI-1640 (PM150110)+15ng/mL G-CSF+10% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio1:2-1:4
Medium Renewalevery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium＋30% FBS＋10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Misc Information
SubculturingCultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2×10^5 viable cells/ml. Maintain cell density between 1×10^5 and 1×10^6 viable cells/ml.
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