tcel225 SW620

A recurrence of the malignancy resulted in a wide-spread metastasis from the colon to an abdominal mass.The established cell line consists mainly of individual small spherical and bipolar cells lacking microvilli.The cells synthesize only small quantities of carcinoembryonic antigen(CEA), and are highly tumorigenic in nude mice.The cells are negative for expression of CSAp(CSAp-) and colon antigen 3, negative.The cells are positive for keratin by immunoperoxidase staining.The line was derived from a metastasis of the same tumor from which the SW480 was derived.There is a G-】 A mutation in codon 273 of the p53 gene resulting in an Arg-】 His substitution.The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes.N-myc oncogene expression was not detected.