tcel194 RF / 6A

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Product Description
Weibel-Palade granules are seen by electron microscopy.

Information
ApplicationLate passage cells have been used as feeder layers to enhance attachment, subcultivation and maintenance of uveal melanocytes.
TissueMonkey choroid
retina
Morphologyepithelial
Growth Propertiesadherent

Specifications
Complete Growth MediumMEM (PM150410)+10% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio1:2-1:4
Medium Renewalevery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium＋30% FBS＋10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Gene Expressionfactor Ⅷ; fibronectin
DurationLate passage cells have been used as feeder layers to enhance attachment, subcultivation and maintenance of uveal melanocytes.

Misc Information
SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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