Product Description
Forms colonies in soft agar and has an abnormal karyotype.Resistant to clinically relevant concentrations of adriamycin, melphalan and cisplatin.Both cultured cells and xenografts exhibit androgen and estrogen receptors.Xenograft models have been used to show that treatment with 17 beta Estradiol can induce progesterone receptors in this human ovarian carcinoma.
Information
Applicationtransfection
OVCAR3 is an appropriate model system in which to study drug resistance in ovarian cancer and the presence of hormone receptors should be useful for the evaluation of hormonal therapy.
TissueHuman ovary
Morphologyepithelial
Growth Propertiesadherent
EffectsYes, Forms colonies in soft agar
Yes, in nude mice inoculated subcutaneously with 10^7 cells. Tumors developed within 21 days at 100% frequency(5/5).
Specifications
Complete Growth MediumRPMI-1640 (PM150110)+20% FBS (164210-500)+0.01mg/mL bovine Insulin+1% P/S (PB180120)
Subcultivation Ratio1:2-1:4
Medium Renewalevery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium＋30% FBS＋10% DMSO
Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5%
Temperature: 37℃
TumorigenicYes
Receptor Expressionandrogen receptor, positive; estrogen receptor, positive; progesterone receptor, positive
Durationtransfection; OVCAR3 is an appropriate model system in which to study drug resistance in ovarian cancer and the presence of hormone receptors should be useful for the evaluation of hormonal therapy.
Misc Information
SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor.
Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.