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Cell Line
MOLT-4
tcel160
MOLT-4
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AVAILABLE SIZES
1×10⁶cells/t25culturebottle
$
0.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
Data sheet
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
Treatment
Temperature
Exposure
Observe Band
Fixative
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Acetone
Formaldehyde
Methanol
Paraformaldehyde
Other
Antigen Retrieval
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Heat mediated
Enzymatic
Other
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Positive Control
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Product Description
The patient had received prior multidrug chemotherapy.There is a G-】 A mutation at codon 248 of the p53 gene.P53 is not expressed.The cells do not produce immunoglobulin or Epstein-Barr virus.The line was originally contaminated with mycoplasma but has been cured with antibiotics.
Information
Application
transfection
Tissue
Human peripheral blood
Morphology
lymphoblast
Growth Properties
suspension
Effects
Yes, in untreated nude mice, anti lymphocyte serum treated mice and X-irradiated mice.
Specifications
Complete Growth Medium
RPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio
1:2-1:4
Medium Renewal
every 2 to 3 days
Cryopreservation
Freeze medium: 60% Basal medium＋30% FBS＋10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Tumorigenic
Yes
Gene Expression
high levels of terminal deoxynucleotidyl transferase(TdT) are produced.
Duration
transfection
Misc Information
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2×10^5 viable cells/ml. Maintain cell density between 1×10^5 and 1×10^6 viable cells/ml.
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