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Cell Line
MCF7 [MCF-7]
tcel149
MCF7 [MCF-7]
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AVAILABLE SIZES
1×10⁶cells/t25culturebottle
$
0.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
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Product Description
The MCF7 line retains several characteristics of differentiated mammary epithelium including ability to process Estradiol via cytoplasmic estrogen receptors and the capability of forming domes. The cells express the WNT7B oncogene.RefGrowth of MCF7 cells is inhibited by tumor necrosis factor alpha(TNF alpha).Secretion of IGFBP's can be modulated by treatment with anti-estrogens.
Information
Application
transfection
Tissue
Human mammary gland, breast
derived from metastatic site: pleural effusion
Morphology
epithelial
Growth Properties
adherent
Specifications
Complete Growth Medium
MEM (PM150410)+0.01mg/mL rh-Insulin (PB180433)+10% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio
1:2-1:4
Medium Renewal
every 2 to 3 days
Cryopreservation
Freeze medium: 60% Basal medium＋30% FBS＋10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Duration
transfection
Misc Information
Subculturing
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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