Product Description
The cells are negative for expression of CSAp(CSAp-) and colon antigen 3.The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes.N-myc and sis oncogene expression were not detected.Tumor specific nuclear matrix proteins CC-3 and CC-4 are expressed.
Information
Applicationtransfection host
TissueHuman colon
derived from metastatic site: left supraclavicular region
Morphologyepithelial
Growth Propertiesadherent
EffectsYes, in nude mice.
Specifications
Complete Growth MediumHam's F-12K (PM150910)+10% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio1:2-1:4
Medium Renewalevery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium＋30% FBS＋10% DMSO
Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5%
Temperature: 37℃
TumorigenicYes
Antigen ExpressionHLA A11, B15, B17, Cw1, Cw3; Blood Type B
Gene Expressioncarcinoembryonic antigen(CEA) 908ng/10^6 cells/10days. The cells are negative for expression of CSAp(CSAp-) and colon antigen 3.
Durationtransfection host
Misc Information
SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor.
Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.