tcel135 L1210

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Data sheet

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Product Description

Tested and found negative for ectromelia virus(mousepox).

Information

TissueMouse peripheral blood
Morphologylymphoblast
Growth Propertiessuspension
EffectsYes, in nude mice.

Specifications

Complete Growth MediumDMEM (PM150210)+10% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio1:2-1:4
Medium Renewalevery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
TumorigenicYes

Misc Information

SubculturingCultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2×10^5 viable cells/ml. Maintain cell density between 1×10^5 and 1×10^6 viable cells/ml.
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