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Cell Line
HEC-1-A
tcel99
HEC-1-A
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AVAILABLE SIZES
1×10⁶cells/t25culturebottle
$
0.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
Treatment
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Acetone
Formaldehyde
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Heat mediated
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Product Description
PAF induces increased expression of c-fos.
Information
Tissue
Human uterus
endometrium
Morphology
epithelial
Growth Properties
adherent
Effects
Yes, in nude mice. The cells form moderately well differentiated adenocarcinomas consistent with endometrial carcinoma(gradeⅡ). Yes, in the cheek pouch of cortisone treated hamsters. The cells form typical papillary adenomas.
Specifications
Complete Growth Medium
McCoy's 5A (PM150710)+10% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio
1:2-1:4
Medium Renewal
every 2 to 3 days
Cryopreservation
Freeze medium: 60% Basal medium＋30% FBS＋10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Tumorigenic
Yes
Receptor Expression
Receptor expression: platelet activating factor(PAF)
Antigen Expression
Blood Type B; Rh＋
Gene Expression
Oncogenes: c-fos＋
Misc Information
Subculturing
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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