tcel60 CHL

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Product Description
This cell line produces about 0.1 mU of t-PA per cell per day.

Information
Applicationtransfection host derived from RPMI 7932 that can grow to a higher density, namely 5×10^7 cells/mL.
TissueHamster skin
Morphologyepithelial
Growth Propertiesadherent

Specifications
Complete Growth MediumDMEM (PM150210)+10% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio1:2-1:4
Medium Renewalevery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium＋30% FBS＋10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Gene Expressionhuman tissue plasminogen activator(t-PA)
Durationtransfection host derived from RPMI 7932 that can grow to a higher density, namely 5×10^7 cells/mL.

Misc Information
SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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