Product Description
Upon reaching confluence, the cells express characteristics of enterocytic differentiation. Ref Caco-2 cells express retinoic acid binding protein I and retinol binding protein Ⅱ.
Information
Applicationtransfection host
TissueHuman colon
Morphologyepithelial
Growth Propertiesadherent
EffectsYes, in nude mice forms moderately well differentiated adenocarcinoma consistent with colonic primary(gradeⅡ).
Specifications
Complete Growth MediumMEM (PM150410)+20% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio1:2-1:4
Medium Renewalevery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium＋30% FBS＋10% DMSO
Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5%
Temperature: 37℃
TumorigenicYes
Receptor ExpressionThis cell line expressed heat stable enterotoxin(Sta, E.coli) and epidermal growth factor(EGF).
Gene Expressionkeratin, retinoic acid binding protein 1, retinol binding protein 2
Durationtransfection host
Misc Information
SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor.
Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.