tcel22 AGS

Order Now


$0.00 ORDERING INFORMATION International

Data sheet

Download PDF Datasheet

Technical Inquries

Submit Review

Product Description

The cells have a plating efficiency of 34% in the medium below. The line was cured at the ATCC of a prior mycoplasma infection . Subsequently, AGS has been determined to be infected with Parainfluenza type 5(PIV5 formerly known as SV5).


Applicationtransfection host
TissueHuman stomach
Growth Propertiesadherent
EffectsYes, in athymic BALB/c mice.


Complete Growth MediumHam's F-12 (PM150810)+10% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio1:3-1:6
Medium Renewalevery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Durationtransfection host

Misc Information

SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Get valuable resources and offers directly to your email.