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Cell Line
769-P
tcel9
769-P
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AVAILABLE SIZES
1×10⁶cells/t25culturebottle
$
0.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
Data sheet
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
Treatment
Temperature
Exposure
Observe Band
Fixative
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Acetone
Formaldehyde
Methanol
Paraformaldehyde
Other
Antigen Retrieval
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Heat mediated
Enzymatic
Other
None
Positive Control
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1:
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Product Description
The cells are globular with indistinct borders, have a high nucleus to cytoplasm ratio and exhibit both microvilli and desmosomes.They can be grown in soft agar.
Information
Tissue
Human kidney
Morphology
epithelial
Growth Properties
adherent
Effects
Yes, forms colonies in soft agar
Yes, forms tumors in immunosuppressed hamsters
Yes, forms tumors in nude mice.
Specifications
Complete Growth Medium
RPMI-1640 (PM150110)+10% FBS (164210-500)+1% P/S (PB180120)
Subcultivation Ratio
1:4-1:8
Medium Renewal
every 2 to 3 days
Cryopreservation
Freeze medium: 60% Basal medium＋30% FBS＋10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Tumorigenic
Yes
Misc Information
Subculturing
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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