tcna10439 UBA2 Antibody / SUMO-activating enzyme subunit 2 / SAE2

Western blot testing of human 1) HeLa 2) placenta, 3) MCF7, 4) A549, 5) SK-OV-3, 6) 22RV1, 7) A431 and 8) COLO320 lysate with UBA2 antibody at 0.5ug/ml. Predicted molecular weight: ~72 kDa but routinely observed at ~90 kDa.Western blot testing of rat 1) brain 2) lung, 3) liver and mouse 4) brain, 5) lung, 6) liver, 7) testis lysate with UBA2 antibody at 0.5ug/ml. Predicted molecular weight: ~72 kDa but routinely observed at ~90 kDa.IHC testing of FFPE human colon cancer tissue with UBA2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.IHC testing of FFPE human breast cancer tissue with UBA2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.IHC testing of FFPE mouse testis tissue with UBA2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.IHC testing of FFPE rat testis tissue with UBA2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Product Description
Ubiquitin-like 1-activating enzyme E1B (UBLE1B) also known as SUMO-activating enzyme subunit 2 (SAE2) is an enzyme that in humans is encoded by the UBA2 gene. Posttranslational modification of proteins by the addition of the small protein SUMO, or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins

Information
ApplicationWB, IHC-P, ELISA
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
Immunogen / Amino acidsA recombinant human protein corresponding to amino acids E449-K564 was used as the immunogen for the SAE2 antibody.
ConjugationAntigen affinity purified
ClonalityPolyclonal
IsotypeRabbit IgG

Specifications
FormLyophilized powder
Storage BufferLyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Recommended DilutionWestern Blot: 0.5-1ug/ml
IHC (FFPE): 1-2ug/ml
Direct ELISA: 0.1-0.5ug/mlOptimal dilution of the UBA2 antibody should be determined by the researcher.

Misc Information
Storage InstructionAfter reconstitution, the UBA2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
SwissProtQ9UBT2
ReferencesAntigen affinity purified
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