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Primary Antibody
MAPKAPK2 Antibody
tcna9890
MAPKAPK2 Antibody
Western blot testing of mouse embryonic fibroblasat lysates from double KO mice (lanes 2 & 3) and rescued by introduction of MK2 gene in second lane. MK2 / MAPKAPK2 antibody was used at 0.5ug/ml. Predicted molecular weight: ~45kDa.
Western blot of HEK293 lysate overexpressing mouse MK2-GFP or MK5-GF probed with MAPKAPK2 antibody at 0.5ug/ml: cat # R36061 on left and cat # R35113 on right. Predicted molecular weight of MAPKAPK2+GFP: ~72kDa.
Immunoprecipitation: MAPKAPK2 antibody (cat # R36061 and cat # R35113, 1.5ug used) precipitates from lysates of MK2/MK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK2 expression through retroviral transduction.
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AVAILABLE SIZES
100ug
$
498.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
Data sheet
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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CELLS
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Product Description
Additional name(s) for this target protein: Mitogen-activated protein kinase-activated protein kinase 2; MK2
Information
Application
WB, IP, ELISA (peptide)
Species Reactivity
Human, Mouse
Host Species
Goat
Immunogen / Amino acids
Amino acids SRVLKEDKERWED were used as the immunogen for this MAPKAPK2 antibody.
Conjugation
Antigen affinity purified
Clonality
Polyclonal
Isotype
Goat Ig
Specifications
Form
Liquid
Storage Buffer
0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
Recommended Dilution
Western blot: 0.5-1.5ug/ml
Immunoprecipitation: 1.5ug
ELISA (peptide) LOD: 1:128000Optimal dilution of the MAPKAPK2 antibody should be determined by the researcher.
Misc Information
Storage Instruction
Aliquot and store the MAPKAPK2 antibody at -20oC.
Gene ID
9261
(human);
References
Antigen affinity purified
related data
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