tcna9794 NQO1 Antibody

Western blot testing of human kidney lysate with NQO1 antibody at 0.03ug/ml. Predicted molecular weight ~30kDa.Western blot of rat (A) and pig (B) kidney lysate with NQO1 antibody at 1ug/ml. Predicted molecular weight ~30kDa.IHC testing of FFPE human kidney tissue with NQO1 antibody at 5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.IF/ICC testing of fixed and permeabilized human HepG2 cells with NQO1 antibody (green) at 5ug/ml and DAPI nuclear stain (blue).
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Product Description
Additional name(s) for this target protein: NAD(P)H dehydrogenase, quinone 1

Information
ApplicationWB, IHC-P, IF/ICC, ELISA (peptide)
Species ReactivityHuman, Rat, Dog
Host SpeciesGoat
Immunogen / Amino acidsAmino acids SIPTDNQIKARK were used as the immunogen for this NQO1 antibody.
ConjugationAntigen affinity purified
ClonalityPolyclonal
IsotypeGoat Ig

Specifications
FormLiquid
Storage Buffer0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
Recommended DilutionWestern blot: 0.03-0.1ug/ml
IHC (FFPE): 5ug/ml
IF/ICC: 5ug/ml
ELISA (peptide) LOD: 1:64000Optimal dilution of the NQO1 antibody should be determined by the researcher.

Misc Information
Storage InstructionAliquot and store the NQO1 antibody at -20oC.
Gene ID1728 (human);
ReferencesAntigen affinity purified
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