tcna8764 HDAC1 Antibody

Western blot testing of HeLa lysate with HDAC1 antibody at 0.5ug/ml. Predicted molecular weight 55~60 kDa. Larger size likely due to sumoylation.ChIP of 2ug of HDAC1 antibody with 1ug MCF7 chromatin using the Chromatrap® spin column sonication kit (Protein G) measuring H3 enrichment onto the p21 locus.Western blot testing of human 1) duodenum and 2) lymph node lysate with HDAC1 antibody at 0.1ug/ml. Predicted molecular weight 55~60 kDa.Western blot testing of mouse NIH3T3 lysate with HDAC1 antibody at 0.1ug/ml. Predicted molecular weight 55~60 kDa. Larger size likely due to sumoylation.
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Product Description
Additional name(s) for this target protein: Histone Deacetylase 1

Information
ApplicationWB, ELISA (peptide)
Species ReactivityHuman, Mouse
Host SpeciesGoat
Immunogen / Amino acidsAmino acids KPEAKGVKEEVK were used as the immunogen for this HDAC1 antibody.
ConjugationAntigen affinity purified
ClonalityPolyclonal
IsotypeGoat Ig

Specifications
FormLiquid
Storage Buffer0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
Recommended DilutionWestern blot: 0.5-1.5ug/ml
ELISA (peptide) LOD: 1:4000Optimal dilution of the HDAC1 antibody should be determined by the researcher.

Misc Information
Storage InstructionAliquot and store the HDAC1 antibody at -20oC.
Gene ID3065 (human);
ReferencesAntigen affinity purified
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