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Primary Antibody
HuD Antibody / ELAVL4
tcna6710
HuD Antibody / ELAVL4
Western blot testing of 1) rat brain, 2) mouse brain and 3) human U87 lysate with HuD antibody. Expected/observed molecular weight ~42 kDa.
IHC testing of FFPE human meningeoma tissue with HuD antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE mouse brain with HuD antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE rat brain with HuD antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
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AVAILABLE SIZES
100ug
$
498.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
Data sheet
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
Treatment
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Product Description
HuD, otherwise known as ELAV-like protein 4 or PNEM, is a protein that in humans is encoded by the ELAVL4 gene. This human gene is located at 1p34 by in situ hybridization. The HuD/ELAVL4 protein is anRNA-binding protein. ELAVL4 has specificity for 3-prime uridylate-rich untranslated regions of growth factor mRNAs. And HuD is expressed only in neurons and it binds toAU-rich element-containing mRNAs. As a result of this interaction the, half-life of the transcript is increased. Also, HuD is important in neurons during brain development and plasticity.
Information
Application
WB, IHC-P
Species Reactivity
Human, Mouse, Rat
Host Species
Rabbit
Immunogen / Amino acids
Amino acids MEPQVSNGPTSNTSNGPSSNNRNCPSPMQTGATTDDSK of human ELAVL4/HuD were used as the immunogen for the HuD antibody.
Conjugation
Antigen affinity purified
Clonality
Polyclonal
Isotype
Rabbit IgG
Specifications
Form
Lyophilized powder
Storage Buffer
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Recommended Dilution
Western blot: 0.1-0.5ug/ml
IHC (Paraffin): 0.5-1ug/mlOptimal dilution of the HuD antibody should be determined by the researcher.
Misc Information
Storage Instruction
After reconstitution, the HuD antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
SwissProt
P26378
References
Antigen affinity purified
related data
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