tcna6035 AChR Antibody (muscarinic)

Western blot testing of AChR antibody and Lane 1: rat brain; 2: mouse brain; 3: U87; 4: SHG-44; 5: Neuro-2a; 6: HeLa cell lysateIHC-P: AChR antibody testing of rat brain tissue
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Data sheet

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Product Description

Muscarinic acetylcholine receptor 1 is a muscarinic receptor. The muscarinic cholinergic receptors belong to a larger family of GPCRs. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. AChR is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract.

Information

ApplicationWB, IHC-P
Species ReactivityHuman, Mouse, Rat
Host SpeciesRabbit
Immunogen / Amino acidsAmino acids 303-317 (KMPMVDPEAQAPTKQ-human) were used as the immunogen for this muscarinic AChR antibody (100% mouse homology).
ConjugationAntigen affinity purified
ClonalityPolyclonal
IsotypeRabbit IgG

Specifications

FormLyophilized powder
Storage Buffer0.5mg/ml if reconstituted with 0.2ml sterile DI water
Recommended DilutionWestern blot: 0.5-1ug/ml
IHC (Paraffin): 0.5-1ug/mlThe stated application concentrations are suggested starting points. Titration of the AChR antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Misc Information

Storage InstructionAfter reconstitution, the AChR antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
SwissProtP11229
ReferencesAntigen affinity purified
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