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Primary Antibody
Connexin 43 Antibody / Cx43
tcna5081
Connexin 43 Antibody / Cx43
IHC-P: Cx43 antibody testing of human lung cancer tissue
IHC-P: Cx43 antibody testing of rat heart tissue
IHC-F testing of rat heart tissue
Western blot testing of 1) human placenta, 2) rat brain, 3) rat heart, 4) mouse brain and 5) mouse heart lysate with Cx43 antibody at 0.5ug/ml. Predicted molecular weight ~43 kDa.
IHC testing of human intestinal cancer tissue with Cx43 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of human breast cancer tissue with Cx43 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of mouse heart tissue with Cx43 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of rat heart tissue with Cx43 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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AVAILABLE SIZES
100ug
$
498.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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CELLS
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Product Description
Connexin 43 (Cx43), also called GAP Junction Protein, alpha, is a member of the connexin gene family and is abundantly expressed in the heart and liver. Connexin 43, the major protein of gap junctions in the heart, is targeted by several protein kinases that regulate myocardial cell-cell coupling. Cx43 also plays a critical role in the physiology of hearing, presumably by participating in the recycling of potassium to the cochlear endolymph.
Information
Application
WB, IHC-P, IHC-F, ICC
Species Reactivity
Human, Mouse, Rat
Host Species
Rabbit
Immunogen / Amino acids
Amino acids 351-367 (HELQPLAIVDQRPSSRA-human) were used as the immunogen for this Cx43 antibody (100% conserved in mouse and rat).
Conjugation
Antigen affinity purified
Clonality
Polyclonal
Isotype
Rabbit IgG
Specifications
Form
Lyophilized powder
Storage Buffer
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Recommended Dilution
Western blot: 0.5-1ug/ml
IHC (Paraffin): 0.5-1ug/ml
IHC (Frozen): 0.5-1ug/ml
Immunocytochemistry: 0.5-1ug/mlTitration of the Cx43 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Misc Information
Storage Instruction
The lyophilized Cx43 antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freezing and thawing.
SwissProt
P17302
References
Antigen affinity purified
related data
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