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Primary Antibody
Recombinant H3K79me1 Antibody / Monomethyl Histone H3 Lysine 79
tcna4788
Recombinant H3K79me1 Antibody / Monomethyl Histone H3 Lysine 79
Western blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2) using recombinant H3K79me1 antibody at 0.5 ug/ml showed a band of Histone H3 monomethylated at Lysine 79 (K79me1) in HeLa cells.
ChIP performed on HeLa cells using recombinant H3K79me1 antibody (5ug). Real-time PCR was performed using primers specific to the gene indicated.
The recombinant H3K79me1 antibody specifically reacts to Histone H3 monomethylated at Lysine 79 (K79me1). No cross reactivity with dimethylated (K79me2), trimethylated (K79me3), or other methylations in histone H3.
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AVAILABLE SIZES
25ug
100ug
$
631.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
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Product Description
This recombinant H3K79me1 antibody reacts to Histone H3 monomethylated at Lysine 79 (K79me1). No cross reactivity with dimethylated (K79me2), trimethylated (K79me3) or other methylations of Histone H3.
Information
Application
WB, ELISA, ChIP
Species Reactivity
All Species
Host Species
Rabbit
Immunogen / Amino acids
A monomethyl-peptide corresponding to Monomethyl-Histone H3 (Lys79) was used as the immunogen for this recombinant H3K79me1 antibody.
Conjugation
Purified
Clonality
Recombinant Rabbit Monoclonal
Clones
RM147
Isotype
Rabbit IgG
Specifications
Form
Liquid
Storage Buffer
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Recommended Dilution
Western Blot: 0.2-1ug/ml
ChIP: 2-10ug/mg of lysate
ELISA: 0.2-1ug/mlThe stated application concentrations are suggested starting points. Titration of the recombinant H3K79me1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Misc Information
Storage Instruction
Store the recombinant H3K79me1 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
SwissProt
P84243
Gene ID
8350
(human);
References
Purified
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