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Primary Antibody
Vimentin Antibody
tcna4645
Vimentin Antibody
Western blot testing of Vimentin-positive cell lines (Molt-4, K562, COS7, Jurkat, HeLa) and Vimentin-negative cell lines (Daudi, Ramos, Raji) using Vimentin antibody at 1:1000. Predicted molecular weight ~58 kDa.
Immunoprecipitation of Vimentin from HeLa cell lysate, and subsequent western blot testing, using Vimentin antibody.
ICC/IF staining of HeLa cells, fixed with 4% Paraformaldehyde, using Vimentin antibody at 1:800.
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AVAILABLE SIZES
0.1ml
$
498.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
Data sheet
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
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UG
CELLS
Treatment
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Fixative
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Acetone
Formaldehyde
Methanol
Paraformaldehyde
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Heat mediated
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Product Description
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Information
Application
WB, ICC/IF, IP
Species Reactivity
Human
Host Species
Mouse
Immunogen / Amino acids
A human recombinant partial protein was used as the immunogen for this Vimentin antibody.
Conjugation
Purified
Clonality
Monoclonal
Clones
4F8-A5-H5
Isotype
Mouse IgG2a
Specifications
Form
Liquid
Storage Buffer
Antibody in PBS with 50% Glycerol and 0.03% ProClin 300
Recommended Dilution
Western blot: 1:1000
ICC/IF: 1:800
Immunoprecipitation: suitableThe stated application concentrations are suggested starting points. Titration of the Vimentin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Misc Information
Storage Instruction
Store the Vimentin antibody at -20oC.
SwissProt
P08670
References
Purified
related data
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