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Primary Antibody
LC3B Antibody (MAP1LC3B)
tcna2826
LC3B Antibody (MAP1LC3B)
Mouse leukaemic monocyte macrophage cells treated with drug U18666A, causing cholesterol and lipid storage in cells, blocking fusion between late endosomes and lysosomes. LC3B antibody detected punctuate staining indicative of autophagic vacuole or phagosome structures.
Fluorescent image of U251 cells stained with LC3B antibody at 1:100. Immunoreactivity is localized to autophagic vacuoles in the cytoplasm.
SY5Y cells were pretreated with 5nM bafilomycin for 24hr and fixed in methanol (left panel) or 4% of paraformaldehyde (right panel). Testing with LC3B antibody at dilution 1:100. Data courtesy of Jianhui Zhu, MD, PhD & Charleen T. Chu, MD, PhD, University of Pittsburgh School of Medicine.
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0.08ml
0.4ml
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208.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
Data sheet
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Application
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Sample
Loading Amount
Loading Type
UG
CELLS
Treatment
Temperature
Exposure
Observe Band
Fixative
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Acetone
Formaldehyde
Methanol
Paraformaldehyde
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Antigen Retrieval
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Heat mediated
Enzymatic
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1:
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Product Description
MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3B is one of the light chain subunits and can associate with either MAP1A or MAP1B. It is a Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). LC3B plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production.
Information
Application
IF, ICC, ELISA
Species Reactivity
Human, Mouse
Host Species
Rabbit
Immunogen / Amino acids
A portion of amino acids 89-122 from the human protein was used as the immunogen for this LC3B antibody.
Conjugation
Antigen affinity purified
Clonality
Polyclonal
Isotype
Rabbit Ig
Specifications
Form
Liquid
Storage Buffer
In 1X PBS pH 7.4 with 0.09% sodium azide
Recommended Dilution
Immunofluorescence: 1:10-1:100
Immunocytochemistry: 1:10-1:50Titration of the LC3B antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Misc Information
Storage Instruction
Aliquot the LC3B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
SwissProt
Q9GZQ8
References
Antigen affinity purified
related data
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