tcna1809 PCNA Antibody

Formalin-fixed, paraffin-embedded human tonsil stained with anti-PCNA antibody (SPM350)Western blot testing of A) human HeLa and B) mouse NIH3T3 cell lysates with anti-PCNA antibody (clone SPM350). Predicted molecular weight ~29kDa, routinely observed at 29~36kDa.
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Product Description
Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases.

Information
ApplicationFACS, IF, WB, IHC-P
Species ReactivityHuman, Monkey, Pig, Mouse, Rat, Chicken, Zebrafish, Drosophila and Yeast. Other species not known.
Host SpeciesMouse
Immunogen / Amino acidsRat PCNA protein was used as the immunogen for this anti-PCNA antibody.
ConjugationUnconjugated
ClonalityMonoclonal
ClonesSPM350
IsotypeMouse IgG2a, kappa

Specifications
FormLiquid
Storage Buffer0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Concentration0.2 mg/ml
Recommended DilutionFlow Cytometry: 0.5-1ug/million cells in 0.1ml
Immunofluorescence: 0.5-1ug/ml
Western blot: 0.5-1ug/ml
Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)The optimal dilution of the anti-PCNA antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer
pH 6.0
for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required)
drip mAb solution onto the tissue section and incubate at RT for 30 min.

Misc Information
Storage InstructionStore the anti-PCNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
SwissProtP12004
ReferencesProtein G affinity chromatography
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