tcna1700 CD79 Antibody

Western blot testing of Ramos cell lysate with CD79 antibody (clone CDLA79a-2).
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$456.00 ORDERING INFORMATION International
TAICLONE BIOTECH CORP.
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+886-2-2735-9682
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Data sheet

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Product Description

A disulphide-linked heterodimer consisting of mb-1 (or CD79a) and B29 (or CD79b) polypeptides, CD79 is non-covalently associated with membrane-bound immunoglobulins on B cells. This complex of mb-1 and B29 polypeptides and immunoglobulin constitute the B cell Ag receptor. CD79a first appears at pre B cell stage, early in maturation, and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. It is not present in myeloid or T cell lines.

Information

ApplicationWB, FACS, IF
Species ReactivityHuman. Other species not tested.
Host SpeciesMouse
Immunogen / Amino acidsAmino acids GTYQDVGSLNIADVQ were used as the immunogen for the CD79 antibody. This sequence is from the cytoplasmic portion of the protein.
ConjugationUnconjugated
ClonalityMonoclonal
ClonesCDLA79a-2
IsotypeMouse IgG3

Specifications

FormLiquid
Storage Buffer0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Concentration0.2 mg/ml
Recommended DilutionFlow Cytometry (intracellular): 0.5-1ug/million cells in 0.1ml
IF: 0.5-1ug/ml for 30 min at RT (1)
Western blot: 1-2ug/mlOptimal dilution of the CD79 antibody should be determined by the researcher.

Misc Information

Storage InstructionStore the CD79 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
SwissProtP11912
ReferencesProtein G affinity chromatography
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