Product Description
S100 belongs to the family of calcium binding proteins. S100 alpha and S100 beta proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the beta-chain (i.e. in S100A and S100B) but not on the alpha-chain of S100 (i.e. in S100A and S100A0). This antibody can be used to localize S100A and S100B in various tissue sections. S100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells. Neoplasms derived from these cells also express S100 protein, albeit non-uniformly. A large number of well-differentiated tumors of the salivary gland, adipose and cartilaginous tissue, and Schwann cell-derived tumors express S100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S100 protein.
Information
ApplicationFACS, IF, WB, IHC-P
Species ReactivityHuman, Mouse, Rat, Cow. Other species not known.
Host SpeciesMouse
Immunogen / Amino acidsRecombinant full-length human protein was used as the immunogen for the S100 beta antibody.
ConjugationUnconjugated
ClonalityMonoclonal
ClonesS100B/1012
IsotypeMouse IgG2a, kappa
Specifications
FormLiquid
Storage Buffer0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Concentration0.2 mg/ml
Recommended DilutionFlow Cytometry: 0.5-1ug/million cells in 0.1ml
Immunofluorescence: 1-2ug/ml
Western blot: 0.5-1ug/ml
Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
Prediluted IHC only format: incubate for 30 min at RT (1)Optimal dilution of the S100 beta antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required)
drip mAb solution onto the tissue section and incubate at RT for 30 min.
Misc Information
Storage InstructionStore the S100 beta antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
ReferencesProtein G affinity chromatography