Product Description
Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain alpha-helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles. Antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin.
Information
ApplicationWB, FACS, IF, IHC-P
Species ReactivityHuman, Mouse, Rat, Hamster, Chicken. Other species not known.
Host SpeciesMouse
Immunogen / Amino acidsRecombinant human Desmin was used as the immunogen for the Desmin antibody.
ConjugationUnconjugated
ClonalityMonoclonal
ClonesDES/1711
IsotypeMouse IgG1, kappa
Specifications
FormLiquid
Storage Buffer0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Recommended DilutionWB: 0.5-1ug/ml
FACS: 0.5-1ug/million cells in 0.1ml
Immunofluorescence: 1-2ug/ml
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RTOptimal dilution of the Desmin antibody should be determined by the researcher.
Misc Information
Storage InstructionStore the Desmin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
ReferencesProtein G affinity chromatography