tcna772saf TGF-alpha Antibody Cocktail- sodium azide free

Formalin-fixed, paraffin-embedded human testicular carcinoma stained with TGF-alpha antibody (TG86 + P/T1)
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$498.00 ORDERING INFORMATION International
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Data sheet

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Product Description

This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this mAb is completely blocked by the peptide used for raising this antibody. TGFa is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

Information

ApplicationFACS, IF, IHC-P
Species ReactivityHuman, Rabbit and Zebrafish. Other species not tested.
Host SpeciesMouse
Immunogen / Amino acidsAmino acids 34-43 (PPVAAAVVSH) from the human protein were used as the immunogen for the TGF-alpha antibody.
ConjugationUnconjugated
ClonalityMonoclonal
ClonesTG86 + P/T1
IsotypeMouse IgMs

Specifications

FormLiquid
Storage Buffer1 mg/ml in 1X PBS; BSA free, sodium azide free
Recommended DilutionFlow Cytometry: 0.5-1ug/million cells in 0.1ml
Immunofluorescence: 1-2ug/ml
Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT (1)Optimal dilution of the TGF-alpha antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer
pH 6.0
for 10-20 min followed by cooling at RT for 20 min.

Misc Information

Storage InstructionStore the TGF-alpha antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
SwissProtP01135
ReferencesPEG precipitation
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