tcna700 Podocalyxin Antibody

Formalin-fixed, paraffin-embedded human angiosarcoma stained with Podocalyxin antibody (2A4).Formalin-fixed, paraffin-embedded human placenta stained with Podocalyxin antibody (2A4).
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Product Description
Podocalyxin/PODXL is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.

Information
ApplicationFACS, IF, IHC-P
Species ReactivityHuman, Rabbit, Rat. Other species not tested.
Host SpeciesMouse
Immunogen / Amino acidsA recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human PODXL was used as the immunogen for the Podocalyxin antibody.
ConjugationUnconjugated
ClonalityMonoclonal
Clones2A4
IsotypeMouse IgM

Specifications
FormLiquid
Storage Buffer0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Recommended DilutionFlow Cytometry: 0.5-1ug/million cells in 0.1ml
Immunofluorescence: 1-2ug/ml
Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the Podocalyxin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer
pH 6.0
for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required)
drip mAb solution onto the tissue section and incubate at RT for 30 min.

Misc Information
Storage InstructionStore the Podocalyxin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
SwissProtO00592
ReferencesPEG precipitation
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