tcna668 Nucleolin Antibody Cocktail

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Product Description

Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This mAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Information

ApplicationFACS, IF, WB, IHC-P
Species ReactivityHuman. Does not react with Mouse, Rat and Cow. Other species not tested.
Host SpeciesMouse
Immunogen / Amino acidsSUDHL1 cell nuclear lysate (364-5) and recombinant human protein (NCL/902) were used as the immunogen for the Nucleolin antibody cocktail.
ConjugationUnconjugated
ClonalityMonoclonal
Clones364-5 + NCL/902
IsotypeMouse IgGs

Specifications

FormLiquid
Storage Buffer0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Recommended DilutionFlow Cytometry: 0.5-1ug/million cells in 0.1ml
Immunofluorescence: 0.5-1ug/ml
Western blot: 0.5-1ug/ml
Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the Nucleolin antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate buffer
pH 6.0
for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required)
drip mAb solution onto the tissue section and incubate at RT for 30 min.

Misc Information

Storage InstructionStore the Nucleolin antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
SwissProtP19338
ReferencesProtein G affinity chromatography
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