tcna448saf GFAP Antibody- sodium azide free

Formalin-fixed, paraffin-embedded human cerebellum stained with GFAP antibody (ASTRO/789).Formalin-fixed, paraffin-embedded human Schwanoma stained with GFAP antibody (ASTRO/789).Formalin-fixed, paraffin-embedded rat cerebellum stained with GFAP antibody (ASTRO/789).SDS-PAGE Analysis of Purified, BSA-Free GFAP Antibody (clone ASTRO/789). Confirmation of Integrity and Purity of the Antibody.
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Product Description
This mAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Information
ApplicationFACS, IF, WB, IHC-P
Species ReactivityHuman, Mouse, Rat, Cow, Pig, Rabbit, Chicken. Other species not tested.
Host SpeciesMouse
Immunogen / Amino acidsRecombinant protein was used as the immunogen for the GFAP antibody.
ConjugationUnconjugated
ClonalityMonoclonal
ClonesASTRO/789
IsotypeMouse IgG1

Specifications
FormLiquid
Storage Buffer1 mg/ml in 1X PBS; BSA free, sodium azide free
Concentration1 mg/ml
Recommended DilutionFlow Cytometry: 0.5-1ug/million cells in 0.1ml
Immunofluorescence: 1-2ug/ml
Western blot: 0.5-1ug/ml
Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the GFAP antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer
pH 6.0
for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required)
drip mAb solution onto the tissue section and incubate at RT for 30 min.

Misc Information
Storage InstructionStore the GFAP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
SwissProtP14136
ReferencesProtein G affinity chromatography
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