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Secondary Antibody
Recombinant Rabbit Anti-Human IgD Antibody
tcna4766
Recombinant Rabbit Anti-Human IgD Antibody
IHC testing of FFPE human lymphoid tissue with recombinant Human IgD antibody. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
Sandwich ELISA of human plasma using recombinant Human IgD antibody as the capture (100ng/well) and biotinylated anti-human light chains (, kappa+, lambda) antibody RM129 as the detect, followed by an alkaline phosphatase conjugated streptavidin.
ELISA of hIgs shows recombinant Human IgD antibody reacts to IgD from human plasma and IgD,, kappa from human myeloma. No cross reactivity with IgG, IgM, IgA, or IgE.
ELISA Titration: the plate was coated with different amounts of human IgD (from plasma). A serial dilution of recombinant Human IgD antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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AVAILABLE SIZES
50ug
100ug
$
598.00
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ORDERING INFORMATION
International
TAICLONE BIOTECH CORP.
order@taiclone.com
+886-2-2735-9682
+886-2-2735-9807
Data sheet
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Western blot (WB)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunocytochemistry (ICC)
Immunoprecipitation (IP)
Co-Immunoprecipitation (CoIP)
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
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Product Description
This recombinant Human IgD antibody reacts to hIgD. No cross reactivity with IgG, IgM, IgA, or IgE.
Information
Application
ICC, IHC, FACS, ELISA
Species Reactivity
Human
Host Species
Rabbit
Immunogen / Amino acids
Human IgD was used as the immunogen for this recombinant Human IgD antibody.
Conjugation
Biotin Conjugate
Clonality
Recombinant Rabbit Monoclonal
Clones
RM123
Isotype
Rabbit IgG
Specifications
Form
Liquid
Storage Buffer
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Recommended Dilution
ELISA: 25ng/well - 200ng/well (Capture)
0.01 - 0.1ug/ml (Detection)
Immunocytochemistry: 0.5-2ug/ml
Immunohistochemistry: 0.5-2ug/mlThe stated application concentrations are suggested starting points. Titration of the recombinant Human IgD antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Misc Information
Storage Instruction
Store the recombinant Human IgD antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
SwissProt
P01880
Gene ID
3495
(human);
References
Biotin Conjugate
related data
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