tcna4762 Recombinant Rabbit Anti-Human IgM Antibody

Sandwich ELISA of human IgM using the recombinant Human IgM antibody as the capture (100ng/well), and biotinylated anti-human light chains (, kappa+, lambda) antibody RM129 as the detect, followed by an alkaline phosphatase conjugated streptavidin.Sandwich ELISA of human plasma using the recombinant Human IgM antibody as the capture (100ng/well), and biotinylated anti-human light chains (, kappa+, lambda) antibody RM129 as the detect, followed by an alkaline phosphatase conjugated streptavidin.ELISA of human immunoglobulins shows the recombinant Human IgM antibody reacts only to human IgM. No cross reactivity with Human IgG, IgE, IgD, or IgA.ELISA Titration: the plate was coated with different amounts of human IgM. A serial dilution of the recombinant Human IgM antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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Product Description
This recombinant Human IgM antibody reacts to the heavy chain of human IgM. No cross reactivity with human IgG, IgA, IgD, or IgE.

Information
ApplicationICC, IHC, FACS, ELISA
Species ReactivityHuman
Host SpeciesRabbit
Immunogen / Amino acidsHuman IgM was used as the immunogen for this recombinant Human IgM antibody.
ConjugationBiotin Conjugate
ClonalityRecombinant Rabbit Monoclonal
ClonesRM121
IsotypeRabbit IgG

Specifications
FormLiquid
Storage Buffer1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Recommended DilutionELISA: 50ng/well - 200ng/well (Capture)
0.05-0.2ug/ml (Detection)
Immunocytochemistry: 0.5-2ug/ml
Immunohistochemistry: 0.5-2ug/ml (1)The stated application concentrations are suggested starting points. Titration of the recombinant Human IgM antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Misc Information
Storage InstructionStore the recombinant Human IgM antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
SwissProtP01871
Gene ID3507 (human);
ReferencesBiotin Conjugate
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