tcna4751saf Recombinant Rabbit Anti-Mouse IgM Antibody- sodium azide free

ELISA of mouse immunoglobulins shows the recombinant Mouse IgM antibody reacts to mIgM; no cross reactivity with IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, human/rat/goat IgG.ELISA Titration: the plate was coated with different amounts of mouse IgM. A serial dilution of recombinant Mouse IgM antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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Product Description
This recombinant Mouse IgM antibody reacts to mIgM. No cross reactivity with IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, human/rat/goat IgG.

Information
ApplicationICC, IHC, IP, FACS, ELISA
Species ReactivityMouse
Host SpeciesRabbit
Immunogen / Amino acidsMouse IgM was used as the immunogen for this recombinant Mouse IgM antibody.
ConjugationPurified
ClonalityRecombinant Rabbit Monoclonal
ClonesRM109
IsotypeRabbit IgG

Specifications
FormLiquid
Storage Buffer1 mg/ml in PBS without glycerol/BSA/sodium azide
Recommended DilutionELISA: 0.005ug/ml - 0.2ug/ml
Immunocytochemistry: 0.5-2ug/ml
Immunohistochemistry (FFPE): 0.5-2ug/ml (1)The stated application concentrations are suggested starting points. Titration of the recombinant Mouse IgM antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Misc Information
Storage InstructionStore the recombinant Mouse IgM antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
SwissProtP01872
Gene ID16019 (human);
ReferencesPurified
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