tcna984pre Mouse Anti-Human IgM Antibody- prediluted

Formalin-fixed, paraffin-embedded human tonsil stained with IgM antibody (DA4-4)
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Product Description

Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin €™s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Information

ApplicationIHC-P
Species ReactivityHuman. Other species not known.
Host SpeciesMouse
Immunogen / Amino acidsHeavy chain human IgM was used as the immunogen for this IgM antibody.
ConjugationUnconjugated
ClonalityMonoclonal
ClonesDA4-4, SA-DA4 or HB57
IsotypeMouse IgG1, kappa

Specifications

FormLiquid
Storage BufferPrediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Recommended DilutionPrediluted IHC only format : incubate for 30 min at RT (2)The optimal dilution of the IgM antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer
pH 6.0
for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required)
drip mAb solution onto the tissue section and incubate at RT for 30 min.

Misc Information

Storage InstructionStore the IgM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
SwissProtP01871 / P20769
ReferencesProtein G affinity chromatography
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