tcna170saf Mouse Anti-Human IgM Heavy Chain Antibody- sodium azide free

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Product Description

This antibody recognizes a protein of 75kDa, identified as the mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. The antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.


ApplicationWB, FACS, IHC-P, IF
Species ReactivityHuman. Other species not known.
Host SpeciesMouse
Immunogen / Amino acidsRecombinant heavy chain of human IgM was used as the immunogen for this antibody.
IsotypeMouse IgG1, kappa


Storage Buffer1 mg/ml in 1X PBS; BSA free, sodium azide free
Concentration1 mg/ml
Recommended DilutionFACS: 0.5-1ug/million cells
IF: 0.5-1ug/ml
WB: 0.5-1ug/ml
IHC (FFPE): 0.5-1ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols
secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer
pH 6.0
for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required)
drip mAb solution onto the tissue section and incubate at RT for 30 min.

Misc Information

Storage InstructionStore the IgM heavy chain antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Gene ID3507 (human);
ReferencesProtein G affinity chromatography
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