tcna4771saf Recombinant Rabbit Anti-Human IgG4 Antibody- sodium azide free

Sandwich ELISA with human plasma using recombinant Human IgG4 antibody as the capture, and biotinylated anti-human light chains (, kappa+, lambda) antibody RM129 as the detect, followed by an AP conjugated streptavidin.Sandwich ELISA with human IgG4 using recombinant Human IgG4 antibody as the capture, and biotinylated anti-human light chains (, kappa+, lambda) antibody RM129 as the detect, followed by an AP conjugated streptavidin.Western blot of human, mouse, rat, and goat IgG shows the recombinant Human IgG4 antibody reacts to hIgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms. No cross reactivity with other isotypes of hIgG, or mouse/rat/goat IgG.IHC testing of FFPE human lymphoid tissue with recombinant Human IgG4 antibody.ELISA Titration: the plate was coated with different amounts of hIgG4. A serial dilution of recombinant Human IgG4 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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Product Description
This recombinant Human IgG4 antibody reacts to the heavy chain of hIgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse/rat/goat IgG.

Information
ApplicationWB, ICC, IHC, FACS, ELISA
Species ReactivityHuman
Host SpeciesRabbit
Immunogen / Amino acidsPeptide corresponding to the hinge region of human IgG4 was used as the immunogen for this recombinant Human IgG4 antibody.
ConjugationPurified
ClonalityRecombinant Rabbit Monoclonal
ClonesRM120
IsotypeRabbit IgG

Specifications
FormLiquid
Storage Buffer1 mg/ml in PBS without glycerol/BSA/sodium azide
Recommended DilutionELISA: 50ng/well - 200ng/well (Capture)
0.05-0.2ug/ml (Detection)
Western Blot: 0.5-2ug/ml
Immunocytochemistry: 1-10ug/ml
Immunohistochemistry: 1-10ug/ml (1)The stated application concentrations are suggested starting points. Titration of the recombinant Human IgG4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Misc Information
Storage InstructionStore the recombinant Human IgG4 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
SwissProtP01861
Gene ID3503 (human);
ReferencesPurified
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