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Product Description
This recombinant Human IgG1 antibody reacts to the heavy chain of human IgG1. No cross reactivity with human IgG2, IgG3, IgG4, IgM, IgA, IgD, IgE, mouse/rat/goat IgG.
Information
ApplicationICC, IHC, FACS, ELISA
Species ReactivityHuman
Host SpeciesRabbit
Immunogen / Amino acidsPeptide corresponding to the hinge region of human IgG1 was used as the immunogen for this recombinant Human IgG1 antibody.
ConjugationBiotin Conjugate
ClonalityRecombinant Rabbit Monoclonal
ClonesRM117
IsotypeRabbit IgG
Specifications
FormLiquid
Storage Buffer1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Recommended DilutionELISA: 50ng/well - 200ng/well (Capture) 0.05-0.2ug/ml (Detection) Immunocytochemistry: 0.5-2ug/ml Immunohistochemistry: 0.5-2ug/ml (1)The stated application concentrations are suggested starting points. Titration of the recombinant Human IgG1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
Misc Information
Storage InstructionStore the recombinant Human IgG1 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
