tcna4768 Recombinant Rabbit Anti-Human IgG1 Antibody

Sandwich ELISA with human IgG1 using recombinant Human IgG1 antibody as the capture, and biotinylated anti-human light chains (, kappa+, lambda) antibody RM129 as the detect, followed by an AP conjugated streptavidin.Sandwich ELISA with human plasma using recombinant Human IgG1 antibody as the capture, and biotinylated anti-human light chains (, kappa+, lambda) antibody RM129 as the detect, followed by an AP conjugated streptavidin.ELISA of hIgGs shows recombinant Human IgG1 antibody only reacted to hIgG1. No cross reactivity with IgG2, IgG3, IgG4, IgE, IgD, IgA, or mouse/rat/goat IgG. ELISA Titration: the plate was coated with different amounts of human IgG1. A serial dilution of recombinant Human IgG1 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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Product Description
This recombinant Human IgG1 antibody reacts to the heavy chain of human IgG1. No cross reactivity with human IgG2, IgG3, IgG4, IgM, IgA, IgD, IgE, mouse/rat/goat IgG.

Information
ApplicationICC, IHC, FACS, ELISA
Species ReactivityHuman
Host SpeciesRabbit
Immunogen / Amino acidsPeptide corresponding to the hinge region of human IgG1 was used as the immunogen for this recombinant Human IgG1 antibody.
ConjugationBiotin Conjugate
ClonalityRecombinant Rabbit Monoclonal
ClonesRM117
IsotypeRabbit IgG

Specifications
FormLiquid
Storage Buffer1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Recommended DilutionELISA: 50ng/well - 200ng/well (Capture)
0.05-0.2ug/ml (Detection)
Immunocytochemistry: 0.5-2ug/ml
Immunohistochemistry: 0.5-2ug/ml (1)The stated application concentrations are suggested starting points. Titration of the recombinant Human IgG1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Misc Information
Storage InstructionStore the recombinant Human IgG1 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
SwissProtP01857
Gene ID3500 (human);
ReferencesBiotin Conjugate
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