tcna4768 Recombinant Rabbit Anti-Human IgG1 Antibody

Data sheet

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Product Description

This recombinant Human IgG1 antibody reacts to the heavy chain of human IgG1. No cross reactivity with human IgG2, IgG3, IgG4, IgM, IgA, IgD, IgE, mouse/rat/goat IgG.

Information

ApplicationICC, IHC, FACS, ELISA
Species ReactivityHuman
Host SpeciesRabbit
Immunogen / Amino acidsPeptide corresponding to the hinge region of human IgG1 was used as the immunogen for this recombinant Human IgG1 antibody.
ConjugationBiotin Conjugate
ClonalityRecombinant Rabbit Monoclonal
ClonesRM117
IsotypeRabbit IgG

Specifications

FormLiquid
Storage Buffer1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Recommended DilutionELISA: 50ng/well - 200ng/well (Capture)
0.05-0.2ug/ml (Detection)
Immunocytochemistry: 0.5-2ug/ml
Immunohistochemistry: 0.5-2ug/ml (1)The stated application concentrations are suggested starting points. Titration of the recombinant Human IgG1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Misc Information

Storage InstructionStore the recombinant Human IgG1 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
SwissProtP01857
Gene ID3500 (human);
ReferencesBiotin Conjugate
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