tcna4749 Recombinant Rabbit Anti-Mouse IgG3 Antibody

ELISA of mouse immunoglobulins shows the recombinant Mouse IgG3 antibody reacts to both mouse IgG3, kappa and IgG3, lambda; No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG.ELISA titration: he plate was coated with different amounts of mouse IgG3. A serial dilution of recombinant Mouse IgG3 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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Product Description
This recombinant Mouse IgG3 antibody reacts to the Fab region of mouse IgG3. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG.

Information
ApplicationWB, ICC, IHC, FACS, ELISA
Species ReactivityMouse
Host SpeciesRabbit
Immunogen / Amino acidsMouse IgG3 was used as the immunogen for this recombinant Mouse IgG3 antibody.
ConjugationBiotin Conjugate
ClonalityRecombinant Rabbit Monoclonal
ClonesRM218
IsotypeRabbit IgG

Specifications
FormLiquid
Storage Buffer1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Recommended DilutionELISA: 0.005-0.2ug/ml
Immunocytochemistry (FFPE): 0.5-2ug/ml
Immunohistochemistry: 0.5-2ug/ml (1)
Western Blot: 0.1-0.5ug/mlThe stated application concentrations are suggested starting points. Titration of the recombinant Mouse IgG3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Misc Information
Storage InstructionStore the recombinant Mouse IgG3 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
SwissProtP03987
Gene ID380795 (human);
ReferencesBiotin Conjugate
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