tcna4748saf Recombinant Rabbit Anti-Mouse IgG2b Antibody- sodium azide free

Data sheet

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Product Description

This recombinant Mouse IgG2b antibody reacts to the Fc region of mIgG2b. No cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human/rat/goat IgG.

Information

ApplicationWB, IP, ICC, IHC, FACS, ELISA
Species ReactivityMouse
Host SpeciesRabbit
Immunogen / Amino acidsMouse IgG2b was used as the immunogen for this recombinant Mouse IgG2b antibody.
ConjugationPurified
ClonalityRecombinant Rabbit Monoclonal
ClonesRM108
IsotypeRabbit IgG

Specifications

FormLiquid
Storage Buffer1 mg/ml in PBS without glycerol/BSA/sodium azide
Recommended DilutionELISA: 0.005ug/ml - 0.2ug/ml
Immunocytochemistry: 0.5-2ug/ml
Immunohistochemistry (FFPE): 0.5-2ug/ml (1)
Western Blot (stronger on non-reduced samples): 0.1-0.5ug/mlThe stated application concentrations are suggested starting points. Titration of the recombinant Mouse IgG2b antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Misc Information

Storage InstructionStore the recombinant Mouse IgG2b antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
SwissProtP01867
Gene ID16016 (human);
ReferencesPurified
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