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Product Description
This recombinant Mouse IgG2a antibody reacts to the Fc region of mIgG2a. No cross reactivity with mouse IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human/rat IgG.
Immunogen / Amino acidsMouse IgG2a was used as the immunogen for this recombinant Mouse IgG2a antibody.
ConjugationPurified
ClonalityRecombinant Rabbit Monoclonal
ClonesRM219
IsotypeRabbit IgG
Specifications
FormLiquid
Storage Buffer1 mg/ml in PBS without glycerol/BSA/sodium azide
Recommended DilutionELISA: 0.005ug/ml - 0.2ug/ml Immunocytochemistry: 0.5-2ug/ml Immunohistochemistry (FFPE): 0.5-2ug/ml (1) Western Blot (non-reduced only): 0.5-2ug/mlThe stated application concentrations are suggested starting points. Titration of the recombinant Mouse IgG2a antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
Misc Information
Storage InstructionStore the recombinant Mouse IgG2a antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
