tcna4746saf Recombinant Rabbit Anti-Mouse IgG2a-Kappa Antibody- sodium azide free

Western blot of nonreduced(-) and reduced(+) mouse IgG2a-, kappa (20 ng/lane), using 0.2ug/ml of recombinant Mouse IgG2a-Kappa antibody. This antibody only reacts to nonreduced Mouse IgG2a-, kappa.ELISA of mouse immunoglobulins shows the recombinant Mouse IgG2a-Kappa antibody reacts to the Fab region of mIgG2a-, kappa; no cross reactivity with IgG2a-lambda, IgG1, IgG3, IgM, IgA, IgE, human/rat/goat IgG.ELISA titer: the plate was coated with different amounts of mouse IgG2a-, kappa. A serial dilution of recombinant Mouse IgG2a-Kappa antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
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Product Description
This recombinant Mouse IgG2a-Kappa antibody reacts to the Fab region of mouse IgG2a-k. No cross reactivity with IgG2al, IgG1, IgG3, IgM, IgA, IgE, human/rat/goat IgG.

Information
ApplicationWB (non-reduced only), IP, ICC, IHC, FACS, ELISA
Species ReactivityMouse
Host SpeciesRabbit
Immunogen / Amino acidsMouse IgG was used as the immunogen for this recombinant Mouse IgG2a-Kappa antibody.
ConjugationPurified
ClonalityRecombinant Rabbit Monoclonal
ClonesRM107
IsotypeRabbit IgG

Specifications
FormLiquid
Storage Buffer1 mg/ml in PBS without glycerol/BSA/sodium azide
Recommended DilutionELISA: 0.005ug/ml - 0.2ug/ml
Immunocytochemistry: 0.5-2ug/ml (1)
Immunohistochemistry (FFPE): 0.5-2ug/ml
Western Blot (non-reduced only): 0.1-0.5ug/mlThe stated application concentrations are suggested starting points. Titration of the recombinant Mouse IgG2a-Kappa antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Misc Information
Storage InstructionStore the recombinant Mouse IgG2a-Kappa antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
SwissProtP01863
Gene ID380793 (human);
ReferencesPurified
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