tcna1726saf Recombinant Rabbit Anti-Human IgG Heavy Chain Antibody- sodium azide free

Data sheet

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Product Description

Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It reacts with all sub-classes of gamma chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Information

ApplicationFACS, IF, IHC-P
Species ReactivityHuman. Other species not known.
Host SpeciesRabbit
Immunogen / Amino acidsHuman Ig Gamma Chain was used as the immunogen for this recombinant IgG antibody.
ConjugationUnconjugated
ClonalityRecombinant Rabbit Monoclonal
ClonesIG507R
IsotypeRabbit IgG, kappa

Specifications

FormLiquid
Storage Buffer1 mg/ml in 1X PBS; BSA free, sodium azide free
Concentration1 mg/ml
Recommended DilutionFlow Cytometry: 0.5-1ug/million cells in 0.1ml
Immunofluorescence: 0.5-1ug/ml
Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Prediluted IHC only format: incubate for 30 min at RT (1)The optimal dilution of the recombinant IgG antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required)
drip mAb solution onto the tissue section and incubate at RT for 30 min.

Misc Information

Storage InstructionStore the recombinant IgG antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
SwissProtP01857
ReferencesProtein A affinity chromatography
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